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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in lots of tumors, which ends up in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was observed to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial purpose in endorsing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two additional splice variants were reported but in-depth nucleotide sequence data is just not however available.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope recognized through the antibody.
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This information outlines additional doable optimization of mobile and immunoassay parameters to be certain the absolute best outcomes are...
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This information outlines more possible optimization of cellular and immunoassay parameters to make sure the absolute best outcomes are...
Antibodies sure to the blocking peptide no longer bind into the epitope thr77 login to the focus on protein. By evaluating the staining in the blocked antibody compared to the antibody by yourself, one can see which staining is particular.
Concentrate on Information Cyclin E belongs towards the hugely conserved cyclin family members, whose associates are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation styles which add towards the temporal coordination of every mitotic celebration. Cyclin E forms a complex with and functions to be a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and it is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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